COVID-19 caused accelerated research into developing vaccine options. Some of the created vaccines are based on mRNA which, if not protected, easily degrades in humans before its therapeutic benefits can be realized.
For chemists performing bench-scale organic synthesis, flash column chromatography is often the primary purification technique. When synthesis needs scaling to multi-gram levels, so does the flash purification. The logical approach is to increase the flash cartridge or column size, but this is only part of the solution. In this article we discuss the process of simplifying flash purification scale-up.
Scaling-up flash chromatography can seem a daunting task but it is a fairly straightforward process once the following parameters are determined: