Welcome to the Biotage Scale Up Blogs.

      Bob Bickler


      Recent Posts

      Flash Chromatographic Purification of Cannabicitran (CBCT)

      Nov 3, 2020 1:49:31 PM / by Bob Bickler posted in Reversed-phase, purification, Cannabinoids

      0 Comments

      Cannabicitran is one of many minor cannabinoids found in cannabis though not much is known about this compound as far as possible medicinal benefits.

      Read More

      What is the Best Way to Isolate CBDA from Hemp?

      Oct 6, 2020 1:44:55 PM / by Bob Bickler posted in CBDA, purification

      0 Comments

      Cannabidiolic acid, or CBDA, has purported analgesic properties relieving pain and inflammation[1]. CBDA is also the starting material for CBD which is produced through a procedure called decarboxylation using heat but which can also degrade CBD generating yield losses[2].

      Read More

      How to efficiently remove water from cannabinoid chromatography fractions

      Sep 8, 2020 2:36:37 PM / by Bob Bickler posted in Cannabis, Reversed-phase, Media and Resin

      0 Comments

      Cannabinoid isolation requires several steps from harvesting the biomass to extraction, winterization, decarboxylation (if desired), distillation, and finally chromatographic purification and isolation of the desired cannabinoids. It is during the chromatography step where water is introduced which, as we all know, can be challenging to remove from the desired cannabinoid by distillation or other evaporative technologies.

      Read More

      How can I determine which cannabinoid I am collecting when using flash chromatography?

      Jul 28, 2020 4:53:06 PM / by Bob Bickler posted in Cannabis, Detector, UV

      0 Comments

      Flash chromatography is the go-to cannabinoid purification technique because of its effectiveness and relative ease of use. This technology can easily remove THC from CBD in a process known as THC remediation. Flash chromatography is also used to separate and isolate other cannabinoids as well (CBN, CBC, CBG, CBDA, CBCT, etc.).

      Read More

      Flash Chromatographic Purification of Cannabichromene (CBC)

      May 20, 2020 2:03:50 PM / by Bob Bickler posted in Cannabis, Reversed-phase, Sfär, Normal Phase

      0 Comments

      Cannabichromene (CBC) is one of the four most prevalent cannabinoids expressed by cannabis plants (Pollastro, 2018). Unlike the psychoactive tetrahydrocannabinol, CBC is benign in this regard, but does have purported health benefits.

      Read More

      CBN Purification by Flash Chromatography

      Apr 30, 2020 5:16:00 PM / by Bob Bickler posted in Cannabis, Reversed-phase

      0 Comments

      Cannabidiol or CBN, is a cannabinoid of interest because of its potential in medicinal applications. However, CBN is not expressed by cannabis plants in high yields and is actually formed from degraded (oxidized) tetrahydrocannabinol (THC).

      Read More

      CBG Purification by Flash Chromatography

      Apr 28, 2020 5:12:36 PM / by Bob Bickler posted in Cannabis, Reversed-phase

      0 Comments

      As one of the major cannabinoids present in cannabis, Cannabigerol (CBG), is gaining interest as possible therapeutic. Though all other cannabinoids are created from CBGA (the acid form of CBG), it is metabolized to CBG and then primarily to THC or CBD, depending on the plant species.

      Read More

      How can I purify CBN from a cannabinoid mixture?

      Mar 10, 2020 4:40:29 PM / by Bob Bickler posted in Cannabis, Chromatography Fundamentals, Reversed-phase

      0 Comments

      In recent years, most of the interest in the medicinal cannabis market  has been focused on CBD (THC-free) and, to a lesser extent, THC. However, the market needs are changing with increasing interest now seen in the so-called minor cannabinoids. In this post, I will focus on cannabinol (CBN).

      Read More

      Minor Cannabinoid Separation by Reversed-phase Flash Chromatography

      May 17, 2019 8:30:07 PM / by Bob Bickler posted in Cannabis, Reversed-phase, Sfär, Selekt

      0 Comments

      Previously, I have posted on a normal-phase flash chromatography method to separate and isolate CBG from a CBD-rich hemp distillate. CBG is just one of many naturally occurring minor cannabinoids of interest in this fast-growing market.

      Read More

      Isolation of some minor cannabinoids using flash chromatography

      Apr 16, 2019 11:02:09 PM / by Bob Bickler posted in Cannabis, Chromatography Fundamentals

      0 Comments

      Cannabis entrepreneurs continually seek to differentiate themselves from others in the market. Some focus on THC while others focus on CBD. What I have seen recently after attending some cannabis-specific conferences is a growing interest in isolating/purifying some of the minor, naturally occurring phytocannabinoids such as CBG and CBDV.

      Read More